The use of fed batch cultivation for achieving high cell densities for the pilot scale production of a recombinant protein (Phenylalanine dehydrogenase) in Escherichia coli. Authors:
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چکیده
The feed rate of substrate during fed-batch fermentation affects many physiological variables such as specific growth rate, cell mass accumulation, substrate feed per cell, substrate feed to maintenance, carbon starvation stress, by-product formation and population segregation. These variables are known, or can be theoretically assumed, to influence protein production. Feeding strategies such as DO-stat (Cutayar and Poillon, 1989; Castan and Enfors, 2000), pH-stat (Robbins and Taylor, 1989) constant feeding (Wong et al., 1998), exponential feeding (Korz et. al., 1995) and linearly changing feeding rate (Wong et al., 1998), have been employed in fed-batch cultures of various recombinant E. coli strains.
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